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A new class of syndromic testing

Guanine provides MOBILE QUANTIFICATION ​of 32 detection targets with numerous capabilities not available with multiplex PCR. Sample-to-results in under 1 hour with digital output transmitted to clinical information systems and AI models​.

Guanine's multi-analyte multi-omics sensor offers unique capabilities including:

• QUANTITATIVE:  Electroactive oligonucleotide tags produce electrical current proportional to target concentration using a a pre-programmed formula like a glucose meter.
• SENSITIVE: Low levels are detected with bigger microparticles that deliver more tags per target and filtering a large sample volume to provide more targets for detection.
• SPECIFIC: High specificity using magnetic microparticles for purification and long 70mer capture probes for higher selectivity. Tags are pre-fabricated into quadruplexes to produce 8-oxoguanine signals that are not affected by guanine signals from targets and capture probes.
• MULTIPLEX: Sensors can have 36 electrodes. Each electrode can use tags with PolyG, PolyA, PolyT and PolyC that generate distinct electrical current peaks at different voltages.
• MINIMAL HANDS-ON TIME AND COST: Using off the shelf 96-well microtiter sensors and microtiter magnetic separation.
• SIMPLE PROTOCOL FOR AUTOMATED CARTRIDGE: POC cartridge can filter samples, lyse cells, capture targets on microparticles that are magnetically separated, form sandwiches on specific electrodes and generate electrochemical oxidation signals. Other processes can be adapted.
• MULTI-OMICS: Configurations for quantifying concentrations or percentages of DNA methylation without bisulfite conversion, DNA methylation for 5mC, 5hmC, 5hfc and 5caC, Histone acetylation and methylation, Histone site-specific enzyme activity and inhibition rates, RNA splicing, Mutation sequences, and Protein expression.
  

Guanine's quantitative
multi-analyte
multi-omics sensor
 
Guanine's sensor quantifies low concentrations by binding targets to a microparticle conjugated with millions of electroactive polyGuanine oligonucleotides. Detection targets can include nucleic acids, proteins, small molecules, cells and redox materials.

DNA Methylation Survey

Guanine Inc is seeking feedback from epigenetic researchers, laboratory specialists and clinicians on the shortcomings of existing DNA methylation methods. Interested parties are invited to complete a survey.

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Small data
precision medicine

Guanine can save thousands of dollars per sample for multi-omics testing using customizable tests along with EHR, patient and environmental data, machine learning and heuristics.

Guanine's multi-analyte multi-omics sensor is integrated into a 96-well microtiter kit or single-use point-of-care cartridge to enable target extraction, capture, purification and sandwich creation. A potentiostat generates a voltammetry scan where the oligonucleotide tags oxidize and produce electrical current. The peak electrical signal is converted to the target concentration using a pre-programmed concentration curve. The output is transmitted to an EHR system and AI algorithm.

Guanine's disruptive platform enables mobile syndromic testing at a fraction of the price of optical technologies

• Mobile electrochemical quantification with a low cost reader similar to a glucose meter
• Signal amplification that attaches millions of electroactive tags to nucleic acid targets and/or protein biomarkers instead of replicating millions of copies with PCR
  
• Enhanced accuracy with filter concentration and magnetic separation  

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Guanine's low cost quantitative mobile data can be a game changer for using AI to diagnose and treat complex illnesses

• Increase ML accuracy and precision by developing and training machine learning models with a wide range of critical inputs that are difficult to detect at low concentrations and expensive to quantitate
• Input data can include pathogens, drug resistance biomarkers, genes, biomarkers, antibodies, cytokines, toxins, prions and drug levels
  
• Low cost mobile reader provides input data for AI diagnosis and treatment

Sepsis is the largest hospital cost and the leading cause of hospital deaths

ER doctors must select antibiotics for sepsis infections in
1 hour but cultures for antibiotic resistance take 3 days

Guanine's technology challenges the status quo

Guanine's upcoming syndromic test will quantify 32 sepsis-causing pathogens
and drug resistance biomarkers, and determine antibiotic dose in 1 hour

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Sepsis detection and treatment at admission reduces hospital stays, healthcare costs and mortality to benefit doctors, hospitals and payers

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Guanine’s products can be adapted for many lucrative market applications

• DIY (Do It Yourself) Syndromic Microtiter Kits
• Licensing and development services
• Input data to create and train healthcare machine learning models

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Infections

• $51 Billion by 2028 (more)
• 3.5% CAGR
• Blood infections, respiratory infections, GI infection, tickborne diseases

Biomarkers

• $104 Billion by 2028 (more)
• 12.0% CAGR
• Cancer, neurological, cardiac

Companion Diagnostics

• $16 Billion by 2029 (more)
• 11.0% CAGR
• Drug selection, efficacy, risk, dose

Healthcare AI

• $194 Billion by 2030 (more)
• 38.1% CAGR
• Precision medicine, chronic therapies
  

Industrial Microbiology

• $32 Billion by 2033 (more)
• 7.6% CAGR
• Food, water, bioreactors, animals

Biodefense

• $33 Billion by 2033 (more)
• 7.3% CAGR
• Air, water, insects, people
  

Guanine's patented detection tag changes biodetection from optical to electrical

Electrochemically detectable oligonucleotide tags (EC-DOTs) rich in guanine, provide a new generation of nucleic acid, protein and small molecule assays. Targets are bound to microparticles conjugated with millions of EC-DOTs. When voltage is applied a burst of electrons produces an oxidation peak proportional to the target’s concentration. This allows fast and accurate quantification. Tests include:

• Detect and quantify 32 nucleic acids and/or proteins and/or small molecules and/or redox targets from a single sample
  
• 100 to 1,000 targets per mL
• Rapid sample preparation in an automated cartridge
• Mobile and inexpensive
• Input to AI/ML

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Guanine has been incubated at the Mount Sinai Health System. Our predecessor company was a spin-off from NASA's Ames Research Center that commercialized NASA’s electrochemical nanosensor for detecting waterborne pathogens.