AMPLIFIED MULTIPLEXING OF NUCLEIC ACID & PROTEIN ANALYTES

Guanine Inc has patented a revolutionary electrochemical detection tag that can be used to amplify and multiplex nucleic acid and protein analytes from the same sample. 

• Signal amplification is achieved by binding analytes with a magnetic particle conjugated with millions of guanine-rich oligonucleotide tags instead of replicating millions of copies.
• Multiplexing is attained by creating multiple analyte sandwiches using multiple analyte-specific particles, multiple working electrodes, and multiple tags employing electroactive G,T,A or C nucleotides.

Guanine signal amplification is simple. Millions of guanine-rich oligonucleotides (synthetic DNA) fit on a microparticle with ligands that capture analytes. The particle-target complexes then form sandwiches on a biosensor electrode. When voltage is applied a burst of electrons is given off to produce a current peak proportional to the target’s concentration. This allows fast and accurate detection of infective pathogens with an inexpensive mobile smart phone potentiostat as used in measuring glucose concentrations.

Amplified multiplex testing is critical for testing many important illnesses particularly those that present with similar symptoms. Unlike other technologies, Guanine can amplify and detect multiple nucleic acid and proteins from the same test to offer rapid, simple and inexpensive detection in a portable device.

MOBILE TESTING WITH AI DIAGNOSIS

Guanine enables fast and accurate detection of infective pathogens with an inexpensive mobile smart phone potentiostat. AI tools can be used with the tests to provide a preliminary diagnosis.

Consumer enters symptoms
on a smart phone, and
AI algorithm recommends
a specific test if warranted

Consumer collects sample
and inserts into cartridge
that is tested with
a smart phone adapter

AI algorithms provide a
preliminary diagnosis for
consumers and doctors who
can treat by telehealth