TECHNOLOGY OVERVIEW
Guanine has invented electrical detection using synthetic DNA tags that will revolutionize accurate, mobile detection of multiple infections.
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TEST PROCESS
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Detection employs simple steps that can be conducted in minutes in a test cartridge that captures targets after onboard lysing, magnetic separation and electrochemical detection.
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Samples are lysed and targets are captured by magnetic particles
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Particle-target complexes are magnetically separated and form sandwiches on electrodes
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Tags composed of majority guanine, adenine, thymine or cytosine generate peaks at different voltage potentials
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QUANTIFY
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Detection tags bind to targets and produce an electrical current peak proportional to the quantity of targets in the sample. The burst is bigger when tags comprise a 20 Guanine sequence that is prefabricated into quadruplexes (planes of 4 guanine separated by Na+ cations) that generate 8-oxoguanine oxidation peaks.
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LIMIT OF DETECTION
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Limit of detection is configured for the intended use since bigger particles bind more tags to produce a stronger signal for measuring lower limit of detection.
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MULTIPLEX
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Voltage generates a current peak if the pathogen is present. A bigger particle with more tags produces a stronger signal for lower limit of detection. A sensor can detect up to 24 targets per sample using 6 working electrodes that can each detect 4 groups of unique tag signals.
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PATENTS
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Guanine has three approved patents for oligonucleotide detection tags, devices, detection methods and artificial intelligence diagnosis.
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US 11,105,801 has patented mobile electrode sandwich detection using single-stranded or quadruplex tags with AI diagnosis and is approved in the US and is pending in Canada and Europe. Download |
US 9,624,532 has patented high throughput detection using single-stranded oligonucleotide tags released to a sensor and is approved in the US, Canada, Germany, France and Great Britain. Download |
US 11,175,285 has patented quadruplex oligonucleotide tags that emit a stronger electrical current than single stranded tags and is patented in the US and Canada. Download |
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TECHNICAL DATA |
Guanine’s test detects carbapenem-resistant bacteria in 45 minutes without an overnight culture |
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Rapid and accurate hybridization from 70 sequences that are not restricted by PCR’s 20 sequence limitation
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Guanine’s assay produced a positive signal in 22/22 samples containing KPC-producing K.pneumoniae and a negative signal in 5/6 non KPC-producing K.pneumoniae samples
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Positive signal peaks are
much higher for targets bound by millions of electrical tags |
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PUBLICATION |
Carbapenem-Resistant Enterobacteriaceae Testing in 45 Minutes using an Electronic Sensor Gordon, N., Bawa, R., Palmateer, G., Rajabi, M., Gordon J.B., Kotb, N.M., Balasubramaniyam, R, Gordon, B.R. Advances in Therapeutics, Diagnostics and Applications. Volume 4, Chapter 26, Jenny Stanford Publishers, Singapore (2022) (in press) Download Universal Bioanalyte Signal Amplification for Electrochemical Biosensor Gordon, N. Biosensors J 5: 135 (2016) Download Highly Sensitive Bacteria Quantification Using Immunomagnetic Separation and Electrochemical Detection of Guanine-Labeled Secondary Beads Jayamohan, H. Gale, B.K,. Minson, B.J., Lambert, C.J., Gordon, N., Sant, H.J. Sensors, 15, 12034–12052 (2015). Download |
