Guanine invented the electrical oligonucleotide detection tag
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Our synthetic DNA detection tags bind to target materials and produce an electrical current signal that is proportional to the quantity of targets in the sample.
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PROCESS
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An automated test process works in a cartridge to capture RNA, antigens, antibodies and/or toxins from a sample or lysed sample with magnetic microparticle conjugates, purify the conjugates by applying a magnetic field to remove nonspecific material that can cause false outcomes, sandwich the microparticle-targets to one of 8 electrodes on the sensor, detect the targets by generating electrical current signals from applying a voltage, then diagnose the infection using AI medical algorithms from the test results, symptoms and other pertinent factors to determine the urgency of seeing a doctor and scheduling a telehealth appointment.
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Capture
Microparticles capture RNA with bound probes or antigens/antibodies with bound antibodies |
Purify
A magnetic field is applied and nonspecific materials are flushed away to reduce false outcomes |
Sandwich
Microparticle-targets are bound to sensor electrodes to detect an individual target or a group of targets |
Detect
An electrical signal is produced on each electrode with a potentiostat to indicate the target presence or concentration |
Diagnose
AI Medical algorithms provide a preliminary diagnosis from test results & symptoms in advance of telehealth confirmation |
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Guanine’s principal detection technologies include: (1) Quadruplex detection tag comprising a guanine sequence that is coiled around a sodium cation (Na+) to form planes of 4 guanine that produce 8-oxoguanine oxidation when voltage is applied, (2) Millions of tags per target caused by streptavidin coated microparticles bound with biotinylated quadruplexes and capture ligands (probes, antibodies or other) to greatly increase the detection signal, (3) Magnetic Microparticles that allow nonspecific materials to be removed when a magnetic field is applied to lessen false detection outcomes and reduce detection noise, (4) Electronic Sensor and Reader which allows microparticle-target complexes to form sandwiches with recognition ligands and apply voltage with an off-the-shelf potentiostat to generate an 8-oxoguanine detection signal.
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ASSAYS
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Guanine tests can measure the best suited pathogen targets from multiples samples including RNA/DNA in a hybridization assay, antigens/antibodies in an immunoassay and toxins in a competitive assay. Targets can be measured individually on a sensor electrode or as a group on a common electrode to rapidly screen a wide range of targets. Multiple electrodes are supplied in an electronic sensor.
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RNA/DNA
Guanine hybridization assay for RNA or DNA |
Microbe/Protein
Guanine immunoassay for microbes or proteins |
Antibodies
Guanine immunoassay for antibodies |
Toxins
Guanine competitive assay for toxins |
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AI TOOLS
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Using our patent pending AI assessment tool, individuals are queried about their symptoms and other information. Based on the information provided, a recommendation is made whether testing is suggested and which tests are most beneficial. A user can test themselves and the results are uploaded to an AI diagnosis tool that applies medical algorithms to assess the test results and symptoms. The test results can recommend the urgency of seeing a doctor or other possible actions. If appropriate a telehealth appointment can be scheduled from the user’s smart phone or computer. Not only is the process faster and less expensive that current conventional methods, individuals are less likely to transmit infections to others.
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Bioanalyte signal amplification and detection with artificial intelligence diagnosis.
Gordon, N. United States Patent and Trademark Office, US 2019/0079084 A1 (2019) View Ultra-sensitive bioanalyte quantification from self-assembled quadruplex tags.. Gordon, N.United States Patent and Trademark Office, US 2018/0106791 A1 (2018) View Ultra-sensitive detection of extremely low level biological analytes using electrochemical signal amplification and biosensor. Gordon, N. United States Patent and Trademark Office, US 9,624,532 B2 (2017) View |
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Carbapenem-Resistant Enterobacteriaceae Testing in 45 Minutes using an Electronic Sensor
Gordon, N., Bawa, R., Palmateer, G., Rajabi, M., Gordon J.B., Kotb, N.M., Balasubramaniyam, R, Gordon, B.R. Current Issues in Medicine: Therapeutics, Diagnostics and Applications. 4 (1), Jenny Stanford Publishers, Singapore (2021) (in press) Highly Sensitive Bacteria Quantification Using Immunomagnetic Separation and Electrochemical Detection of Guanine-Labeled Secondary Beads Jayamohan, H. Gale, B.K,. Minson, B.J., Lambert, C.J., Gordon, N., Sant, H.J. Sensors, 15, 12034–12052 (2015). View |
